Reads Filter Settings

_images/ReadsFilterSettings.png _images/AdvancedReadsFilterSettings.png

Porechop Settings [1] (Optional)

Set Porechop options.

Note

  • Select “I want to trim adapter” if you want to use Porechop to trim adapters. Defaut: selected.
  • Select “I want to skip splitting reads based on middle adapters” if you do not want to splt reads when an adapter is found in the middle. Default: not selected.

Read Score [2] (Required)

Set a minimum average read quality score to filter the reads.

Note

  • Default: 9.

Read Length [2] (Required)

Set a minimum read length to filter reads.

Note

  • Default: 500.

Head Crop [2] (Required)

Set n nucleotides to be trimmed from start of read.

Note

  • Default: 50.
[1]Porechop https://github.com/rrwick/Porechop
[2](1, 2) NanoFilt https://github.com/wdecoster/nanofilt